Shoshi, Astrit: Magnetic lab-on-a-chip for cell analysis : magnetoresistive-based real-time monitoring of dynamic cell-environment interactions. 2013
Inhalt
- GMR theory
- Cell biology
- Real-time monitoring techniques
- Refractive optical microscopy
- Interference reflection microscopy (IRM)
- Total internal reflection fluorescence microscopy (TIRFM)
- Surface plasmon resonance microscopy (SPRM)
- Whispering gallery mode resonance (WGMR)
- Quartz crystal microbalance (QCM)
- Capacitive sensing
- Magnetoresistive sensing
- Magnetic lab-on-a-chip (MAGLab)
- Cell cultures
- Superparamagnetic particles (beads)
- Bead structure and magnetic cell-chip requirements
- Beads for on-chip real-time monitoring and off-chip phagocytosis
- Magnetoresistive biochip
- GMR-sensor layout and magnetic characteristics
- Magnetoresistive bead detection principle
- Dependence of the sensor signal on bead coverage
- Simulations
- MAGLab system
- Polydimethylsiloxane (PDMS) microfluidics
- Construction and setup
- Magneto-transport measurements
- LabView computer control
- FEMLAB simulation of magnetic forces in inhomogeneous fields
- 3D magnetic manipulation of beads
- 3D magnetic manipulation of bead-loaded cells
- MAGLab operation at molecular level: sample analysis principle
- Summary
- Basic cell-environment interactions
- Real-time monitoring of bead phagocytosis during cell spreading
- GMR-based real-time cell phagocytosis monitoring
- Concept of GMR-based real-time cell phagocytosis monitoring
- Real-time cell phagocytosis monitoring
- Summary
- On-chip real-time monitoring of cell migration and detachment
- Summary and outlook
- Appendix
- Preparation and analytical tools
- Thin film deposition
- Optical UV-lithography
- Etching techniques
- Chip fabrication
- Scanning electron microscopy
- Zeta potential
- Evaluation of the sensor bead-surface coverage
- Cell biology
- References
- Acknowledgments
- Curriculum vitae