Muthuramalingam, Meenakumari; Matros, Andrea; Scheibe, Renate; Mock, Hans-Peter; Dietz, Karl-Josef: The hydrogen peroxide-sensitive proteome of the chloroplast in vitro and in vivo. In: Frontiers in Plant Science. Jg.4. 2013, S. 54-1
Inhalt
- Growth of Arabidopsis thaliana and chloroplast isolation
- DTNB-based quantification of thiol groups
- Sample preparation (In vitro and In vivo oxidation treatment)
- Detection of redox-regulated proteins via a sequential labeling strategy
- Streptavidin affinity purification
- Identification of proteins using maldi MS analysis
- Biotin quantification assay
- Results
- Purity of the chloroplast fraction
- Total protein thiol determination
- Effect of H2O2-mediated oxidation on stroma thiol proteins
- Purification and identification of biotinylated proteins following H2O2-mediated oxidation
- Purification and identification of biotinylated proteins following MV-mediated oxidation
- Discussion
- Functional classification of identified proteins
- Redox modification of proteins upon oxidative stress In vivo
- Conclusion
- Acknowledgments
- References
- Appendix