Viefhues, Martina: Analysis and preparation of (bio)nanoobjects in nano-microfluidic devices. 2012
Inhalt
- Introduction
- Theory
- DNA
- Gelelectrophoresis and electrophoretic mobility shift assays
- Electrokinetics and hydrodynamics
- Scaling laws and hydrodynamics on the m-scale
- The electrical double layer
- Electrophoresis and electroosmosis
- Dielectrophoresis
- Intermolecular interactions
- Brownian motion and diffusion
- Kramers rate and first mean passage time
- Materials and methods
- Biomolecular methods
- Production of masterwafer
- Preparation of microfluidic chip
- Experimental setup
- Experimental procedures
- AFM imaging of DNA/RNAP-complexes
- Results and discussion
- Production of device
- Electrostatic sieving of nanoobjects
- Concept of dielectrophoretic mixing and separation
- Dielectrophoretic nanobead manipulation
- DNA manipulation and characterization by dielectrophoresis
- Summary and Outlook
- Appendix
- Results of single DNA experiments
- AFM images of nanobeads
- List of Publications
- Contributions to Conferences
- Bibliography