Vorhölter, Frank-Jörg; Wiggerich, Heinrich-Günter; Scheidle, Heiko; Sidhu, Vishaldeep Kaur; Mrozek, Kalina; Küster, Helge; Pühler, Alfred; Niehaus, Karsten: Involvement of bacterial TonB-dependent signaling in the generation of an oligogalacturonide damage-associated molecular pattern from plant cell walls [...]. In: BMC Microbiology. Jg.12 H. 1. 2012
Inhalt
- Conclusions
- Background
- Results
- Genomic analysis of X. campestris pv. campestris TonB core genes
- The exbD2 gene is involved in pectate lyase activity
- Pectate lyase activity is required for HR on C. annuum
- Elicitor-activity upon co-incubation of X. campestris pv. campestris with C. annuum cell wall material
- Evidence that the newly formed elicitor is an oligogalacturonide DAMP
- Elicitor activity of pectate fragments in N. tabacum and C. annuum cell suspension cultures
- Discussion
- Conclusions
- Methods
- Cultivation of Xanthomonas campestris pv. campestris
- Cultivation of C. annuum plants
- Analysis of enzyme activity
- Genome analysis and recombinant DNA procedures
- Isolation of plant cell wall material
- Co-incubation of X. campestris pv. campestris and C. annuum cell wall material
- Removing LPS from the samples via polymyxin B agarose
- Identification, isolation and characterization of oligosaccharides
- MALDI-TOF MS of isolated oligosaccharides
- Determination of the oxidative burst reaction in plant cell suspension cultures
- Chemicals
- Additional files
- Competing interests
- Authors’ contributions
- Acknowledgements
- Author details
- References