Busche, Tobias; Silar, Radoslav; Picmanova, Martina; Patek, Miroslav; Kalinowski, Jörn: Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory [...]. In: BMC Genomics. Jg.13 H. 1. 2012
Inhalt
- Background
- Results
- The sigH gene and the rshA gene encoding an &b_k;anti-&e_k;&b_k;sigma&e_k; factor of SigH form an operon
- Genes of the sigH-rshA operon are transcribed from multiple promoters of different types
- Global transcriptional profiling of the rshA deletion mutant revealed the majority of known &b_k;SigH-&e_k;&b_k;dependent&e_k; genes and novel ones
- Differential transcription of selected &b_k;SigH-&e_k;&b_k;dependent&e_k; genes was validated by quantitative &b_k;real-&e_k;&b_k;time&e_k; RT-PCR
- Experimental localization of &b_k;SigH-&e_k;&b_k;dependent&e_k; promoters and derivation of a consensus sequence
- Discussion
- The sigH-rshA operon in C. glutamicum exhibits complex transcriptional organization including autoregulation
- Expression analysis of the rshA mutant strain validated and extended the known SigH regulatory network
- Conclusions
- Methods
- Bacterial strains, plasmids, oligonucleotide primers, media and growth conditions
- DNA isolation, manipulation and transfer
- Construction of defined deletions in the C. glutamicum chromosome
- Construction of plasmids
- RNA isolation and quantitative &b_k;real-&e_k;&b_k;time&e_k; RT-PCR
- Microarray hybridization
- Northern blot analysis
- Primer extension analysis
- Chloramphenicol acetyltransferase (CAT) assay
- Additional files
- Competing interests
- Authors´ contributions
- Acknowledgements and funding
- Author details
- References