Heider, Sabine; Peters-Wendisch, Petra; Wendisch, Volker F.: Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum. In: BMC Microbiology. Jg.12 H. 1. 2012
Inhalt
- Results
- Bioinformatical analysis of the carotenogenic genes
- Analysis of the transcriptional organization of the carotenogenic gene clusters
- Gene deletion and complementation analysis of the carotenogenic gene clusters in C. glutamicum
- Enhancing lycopene production by overexpression of carotenogenic genes in the lycopene accumulating strain C. glutamicum ΔcrtEb
- Discussion
- Conclusion
- Methods
- Bacterial strains, media and growth conditions
- Recombinant DNA work
- Determination of the transcriptional start point of crtE and crtB2
- Reverse transcription (RT) for the analysis of transcription units
- Overexpression of carotenogenic genes from C. glutamicum
- Deletion of carotenogenic genes in C. glutamicum WT
- Extraction of carotenoids from bacterial cell cultures
- Analysis of carotenoids
- Computational analysis
- Additional files
- Competing interests
- Authors’ contributions
- Acknowledgements
- References