Schneider, Jens; Peters-Wendisch, Petra; Stansen, K. C.; Götker, Susanne; Stanislav, Maximov; Krämer, Reinhard; Wendisch, Volker F.: Characterization of the biotin uptake system encoded by the biotin-inducible bioYMN operon of Corynebacterium glutamicum. In: BMC Microbiology. Jg.12 H. 6. 2012
Inhalt
- Results
- Influence of biotin on global gene expression in wild type C. glutamicum
- Transcriptional organisation of the putative bioYMN operon
- Characterization of biotin uptake by BioYMN
- Effect of bioYMN overexpression on L-glutamate production triggered by biotin-limitation
- Discussion
- Conclusions
- Methods
- Bacterial strains, plasmids, oligonucleotides, and culture conditions
- Determination of amino acid concentrations
- Construction of plasmids and strains
- Comparative transcriptome analysis using DNA microarrays
- RT-PCR analysis and 5’-RACE
- Transport assays
- Acknowledgements
- Author details
- Authors' contributions
- References