Michel, Klaus-Peter; Schröder, Ann-Kristin; Zimmermann, Maike; Brandt, Sonja; Pistorius, Elfriede K.; Frankenberg-Dinkel, Nicole; Staiger, Dorothee: The hybrid histidine kinase Slr1759 of the cyanobacterium Synechocystis sp. PCC 6803 contains FAD at its PAS domain. In: Archives of Microbiology. Jg.191 H. 6. 2009, S. 553-559
Inhalt
- Bacterial strains and DNA manipulation
- Expression and puriWcation of Trx-His6- and GST-His6-tagged truncated Slr1759 variants
- [c-32P] ATP autophosphorylation assay
- Determination of FAD
- Linear tetrapyrrole binding
- Results and discussion
- Recombinant Slr1759 harbours a Xavin cofactor in its Wrst PAS domain
- The Xavin cofactor is FAD
- Autophosphorylation of Slr1759
- The GAF domain of Hik14 does not bind linear tetrapyrrole
- Conclusion
- References