Lenger, Janina: Activity-based sulfatase and affinity-based metalloprotease probes: ready for take-off. 2011
Inhalt
- Abstract
- Introduction to Activity- and Affinity-Based Proteomics
- Targeting Sulfatases
- Introduction to Sulfatases
- Objectives
- Synthesis of Sulfatase Probes
- Quinone Methide Precursors
- Cyclic Sulfamate Probes
- Azido Reporter Groups and in situ CuAAC
- Conclusion of Sulfatase Probe Synthesis
- Biochemical Evaluation of Quinone Methide Type Probes
- Cyclic Sulfamates as Sulfatase Targeting Groups
- Inhibition Experiments
- Investigation of the Inhibitiory Mechanism
- Preliminary Sulfatase Labeling Results
- Conclusion of Cyclic Sulfamate Probe Investigations
- Experimental
- Expression and Affinity-Based Labeling of Arabidopsis thaliana MMPs
- Introduction to Matrix Metalloproteases
- Objectives
- Development of Covalently-Binding Marimastat Probes
- Transient At-MMP Expression
- Photochemical Affinity-Based Labeling of At-MMPs
- Conclusion
- Experimental Section
- General Experimental Section
- Synthesis: Analytical Methods, Solvents, and Reagents
- Biochemical Material
- General Biochemical Methods
- Conclusion