Schnepel, Jörn: Klonierung, Expression und Charakterisierung kryptischer Fibronektin-Proteinasen. 2000
Inhalt
- Danksagung
- Inhaltsverzeichnis
- 2. Einleitung 4
- 3. Zielsetzung 22
- 4. Zusammenfassung der Ergebnisse 24
- 6.2.1. Genexpression in E. coli-Zellen 50
- OD Optische Dichte
- V Val Valin
- 2. Einleitung
- 3. Zielsetzung
- 4. Zusammenfassung der Ergebnisse
- [INVaF‘]
- [INVaF‘]
- [INVaF‘]
- [INVaF‘]
- [INVaF‘]
- Autoklav Webeco Modell CS-Lab/IDK, Webeco, Lüdenscheid
- Gelatin Serva Feinbiochemica GmbH & Co. KG, Heidelberg
- Albumin 67.000 Da
- Blotpuffer : 25 mM Tris/HCl pH 8,7
- Mca-Peptid : 100 µg/ml Mca-Peptid in 15 % (v/v) DMSO
- 8. Diskussion und Ausblick
- Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., Watson, J.D. (1989) Molecular biology of the cell. Garland Publishing Inc., New York, London, 802-824.
- Aznavoorian, S., Stracke, M.L., Krutzsch, H., Schiffmann, E., Liotta, L.A., Signal transduction for chemotaxis and haptotaxis by matrix molecules in tumor cells (1990) J. Cell Biol. 110, 1427-1438.
- Barret, A.J., Proteolytic enzymes: serin and cystein peptidases (1994) Methods Enzymol. 244.
- Barret, A.J., Proteolytic enzymes: aspartatic and metallo peptidases (1995) Methods Enzymol. 248.
- Carnemolla, B., Cutolo, M., Castellani, P., Balza, E., Raffanti, S., Zardi, L., Characterization of synovial fluid fibronectin from patients with inflammatory diseases and and healthy subjects (1984) Arthtritis Rheum. 27, 913-921.
- Greiling, D., Clark, R.A.F., Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix (1997) J. Cell. Sci. 110, 861-870.
- Heussen, C., Dowdle, E.B., Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates (1980) Anal.Biochem. 102, 196-202.
- Homandberg, G.A., Hui, F., Wen, C., Association of proteoglycan degradation with catabolic cytokine and stromelysine release from cartilage cultured with fibronectin fragments (1986) Arch. Biochem. Biophys. 334, 325-331.
- Hooper, N.M., Families of zinc metalloproteinases (1994) FEBS Letters 354, 1-6.
- Imhoff, J.M., Blondeau, X., Planchenault, T., Emod, I., Collagenase activation of latent matrix-degrading proteinases from human plasma fibronectin (1990) Biol. Chem. Hoppe-Seyler 371, 137-144.
- Maniatis, T., Fritsch, E.F., Sambrook, J., Molecular cloning: A laboratory manual (1989), Cold Spring Harbor Press, Cold Spring Harbor, New York.
- Nickeleit, V., Kaufman, A.H., Zagachin, L., Dutt, J.E., Foster, C.S., Colvin, R.B., Healing corneas express embryonic fibronectin isoforms in the epithelium, subepithelial stroma and endothelium (1996) Am. J. Pathol. 149, 549-558.
- Wennerberg, K., Lohikangas, L., Gullberg, D., Johansson, S., Fässler, R., ß1 Integrin-dependent and -independent polymerization of fibronectin (1996) J. Cell Biol. 132, 227-238.
