Rubiano Castellanos, Claudia Consuelo: Nuclear export signals (NESs) in Arabidopsis thaliana : development and experimental validation of a prediction tool. 2010
Inhalt
- Motivation and overview
- Background
- Nucleo-cytoplasmic transport
- Nuclear protein transport mediated by importin -like receptors
- Nuclear export signals (NESs)
- NESs in the regulation of nuclear transport
- Nuclear export in Arabidopsis thaliana
- Computational prediction of NESs
- Methods
- Development of a prediction tool for NESs
- Establishment of data sets
- Exploratory analysis
- Feature calculation
- Feature selection and data pre-processing
- Training of classifiers
- Performance evaluation criteria
- Pipeline construction
- Prediction on new protein sequences
- Experimental assessment of the nuclear export activity
- Results
- Development of a prediction tool for NESs
- Exploratory analysis
- Tuning and training of classifiers
- Variable importance
- Classifier assessment and selection
- Classification of new samples
- Experimental assessment of the nuclear export activity
- Discussion
- Analysis of LR-NESs of proteins from Arabidopsis
- Development of the prediction tool for LR-NESs
- Analysis of the predicted Arabidopsis LR-NES-containing proteins
- Conclusions and outlook
- Oligonucleotide sequences
- List of Abbreviations
- Bibliography