Kato, Osamu; Youn, Jung-Won; Stansen, K. C.; Matsui, Daisuke; Oikawa, Tadao; Wendisch, Volker F.: Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate. In: BMC Microbiology. Jg.10 H. 1. 2010
Inhalt
- Methods
- Bacterial strains, plasmids, oligonucleotides, and culture conditions
- Determination of glucose and D/L-lactate concentrations
- D-lactate dehydrogenase assay
- Construction of plasmids and strains
- Disruption of dld
- Homologous overexpression of dld
- Expression of dld from C. glutamicum ATCC 13032 in Escherichia coli BL21 (DE3)
- Expression of dld in E. coli BL21 (DE3) and protein purification
- Comparative transcriptome analysis using DNA microarrays
- Results
- Cg1027 encodes D-lactate dehydrogenase
- Dld is required for utilization of D-lactate
- Dld activities under various growth conditions
- DNA microarray analysis of D-lactate specific gene expression changes
- Heterologous expression of dld from C. glutamicum ATCC 13032 in C. Efficiens
- Discussion
- Conclusion
- Acknowledgements
- Author details
- Authors' contributions
- References