Nematollahi, Ghazaleh; Kianianmomeni, Arash; Hallmann, Armin: Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri. In: BMC Genomics. Jg.7 H. 1. 2006
Inhalt
- Results
- Target genes for differential analysis
- Characteristics of genes within subset A
- Characteristics of genes within subset B
- Characteristics of genes within subset C
- Characteristics of genes within subset D
- Characteristics of genes within subset E (novel Volvox genes)
- Characteristics of genes within subset F (novel Volvox genes)
- Characteristics of genes within subset G (novel Volvox genes)
- Selection of primer combinations and testing by genomic PCR and RT-PCR
- Analysis of cell-type specific gene expression by real-time RT-PCR experiments
- Discussion
- Gene subsets A-C: comparison of the obtained real-time RT-PCR results with the expectations
- Gene subsets D-G: validation of the obtained real-time RT- PCR results
- Conclusion
- Methods
- Culture conditions
- Sequence analysis and homology search
- Primer design
- Large-scale separation of cell types
- Isolation of total RNA
- Isolation of genomic DNA
- Genomic PCR
- Standard RT-PCR
- Real-time RT-PCR
- Analysis of gene expression by using the 2-DDCt method
- GenBank accession numbers
- Abbreviations
- Authors' contributions
- Acknowledgements
- References