Valverde, Claudio; Livny, Jonathan; Schlüter, Jan-Philip; Reinkensmeier, Jan; Becker, Anke; Parisi, Gustavo: Prediction of Sinorhizobium meliloti sRNA genes and experimental detection in strain 2011. In: BMC Genomics. Jg.9 H. 1. 2008
Inhalt
- Background
- Methods
- First set of predicted sRNA-encoding genes in S. meliloti chromosomal intergenic regions
- Northern blot detection of the first set of sRNA candidates
- Microarray detection of sRNA candidates
- Novel method for in silico identification of sRNA candidate genes
- Results & Discussion
- A first selection of chromosomal intergenic regions potentially encoding sRNAs
- Experimental verification of selected sRNA candidates in S. meliloti strain 2011
- Growth phase and stress conditions influence accumulation of detected transcripts
- Improvement of the bioinformatic predictive method and application to S. meliloti chromosome
- Putative sRNA-encoding genes in S. meliloti megaplasmids
- Comments on the global scoring procedure
- Conclusion
- Authors' contributions
- Additional material
- Acknowledgements
- References