Pasdziernik, Markus; Kaltschmidt, Barbara; Kaltschmidt, Christian; Klinger, Claudia; Kaufmann, Michael: On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. In: BMC Research Notes. Jg.2 H. 1. 2009, S. 102 ff.
Inhalt
- Methods
- Eucaryotic cell culture and transfections
- Silencing HCR-NTPase in SH-SY5Y by RNA interference
- Overexpressing HCR-NTPase in SH-SY5Y cells
- Construction of a E114A-HCR-NTPase mutant in pRc/ CMV
- Inhibition of apoptosis
- Bioinformatics
- Results
- Loss-of-function: silencing HCR-NTPase by RNA interference
- Gain-of-function: overexpressing HCR-NTPase in SH- SY5Y
- Rescue of SH-SY5Y cells by RNA interference
- The E114A-HCR-NTPase mutant
- Competing interests
- Authors' contributions
- Acknowledgements
- References