Dittami, Simon M.; Scornet, Delphine; Petit, Jean-Louis; Ségurens, Béatrice; Da Silva, Corinne; Corre, Erwan; Dondrup, Michael; Glatting, Karl-Heinz; König, Rainer; Strerck, Lieven; Rouzé, Pierre; Van de Peer, Yves; Cock, J. Marc; Boyen, Catherine; Tonon, Thierry: Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to [...]. In: Genome Biology. Jg.10 H. 6. 2009, S. R66 ff.
Inhalt
- Background
- Results
- Determination of sub-lethal stress conditions
- Intracellular osmolarity and Na+ concentration
- The E. siliculosus microarray represents 17,119 sequences
- cDNA synthesis and amplification provided consistent results with both mRNA and total RNA samples
- Validation of microarray results using quantitative PCR
- Ribosomal protein genes are among those whose transcript abundances are least affected by stress
- Classification of stress response genes using automatic annotations
- Manual classification of stress response genes with the most significant changes in expression
- Stress response genes with unknown functions
- Known brown algal stress genes
- Clusters of genes with similar expression patterns
- Discussion
- Comparison of stress conditions
- Down-regulation of growth and primary metabolism
- Activation of protein degradation, energy stores, and nutrient recycling
- Activation of signaling pathways
- Synthesis of 'classical' stress response proteins
- Ions and potential osmolytes
- Stress response genes with unknown functions
- Conclusions
- Materials and methods
- Growth conditions, stress treatments, and measurements of osmolarity and Na+ concentration
- Sequence preparation and array design
- Automatic annotation and correspondence table
- Sample preparation, hybridization and verification
- Statistical analysis
- Stress response genes with unknown functions
- Data deposition
- Abbreviations
- Authors' contributions
- Additional data files
- Acknowledgements
- References