Kluge, C; Seidel, Thorsten; Bolte, S; Sharma, S. S.; Hanitzsch, M; Satiat-Jeunemaitre, B; Ross, J; Sauer, Markus; Golldack, Dortje; Dietz, Karl-Josef: Subcellular distribution of the V-ATPase complex in plant cells, and in vivo localisation of the 100 kDa subunit VHA-a within the complex. In: BMC Cell Biology. Jg.5 H. 1. 2004, S. 29 ff.
Inhalt
- Background
- Results
- Primary structure of VHA-a
- Detection of VHA-a in membrane preparations in vitro
- Immunochemical analysis of VHA-distribution
- Fluorescence resonance energy transfer between VHA- subunits in vivo
- Discussion
- Conclusions
- Methods
- Plant growth
- Membrane isolation
- Gel electrophoresis and Western blot detection
- Immunoprecipitation
- Anti-VHA- a antibody preparation and other antibodies used in this study
- Construction of fusions between VHA subunits and variants of green fluorescence protein (GFP)
- Protoplast isolation and transformation methods
- Immuno-fluorescence labelling and image acquisition by confocal laser scanning microscopy (CLSM)
- Immunogold-labelling and electron microscopy
- Confocal microscopy of GFP-fusion proteins and FRET- measurement
- Abbreviations
- Authors' contributions
- Acknowledgements
- References