Brockmann-Gretza, Olaf; Kalinowski, Jörn: Global gene expression during stringent response in Corynebacterium glutamicum in presence and absence of the rel gene encoding (p) ppGpp synthase. In: BMC Genomics. Jg.7 H. 1. 2006, S. 230 ff.
Inhalt
- Background
- Results
- Global gene expression in the exponential growth phase is highly different in a C. glutamicum rel-proficient strain and a rel-deficient mutant unable to produce (p)ppGpp
- The mRNA levels of serine and histidine biosynthesis genes in rel-proficient C. glutamicum cultures treated with serine hydroxamate are induced and reach a maximum ten minutes after induction of the stringent response
- Comparison of transcriptional profiles of C. glutamicum rel-proficient and rel-deficient mutant strains before and after induction of the stringent response allowed to identify rel-dependent genes
- Class A genes are under (p)ppGpp mediated stringent control as they were found to be regulated SHX-treatment only in the rel-proficient RES167 strain but not in its rel- deficient derivative RES167Drel
- Class B genes are regulated Rel-independently and show a change in their expression in the rel-proficient strain and the rel-deficient mutant
- Class C genes belong to a variety of functional complexes and show differential regulation only in the rel-deficient mutant
- Discussion
- Materials and methods
- Bacterial strains and growth conditions
- Total RNA isolation from C. glutamicum
- cDNA synthesis, DNA microarray experiments and data analysis
- Real-time RT-PCR assays
- Authors' contributions
- Additional material
- Acknowledgements
- References