Larisch, Christof; Nakunst, Diana; Hueser, Andrea T.; Tauch, Andreas; Kalinowski, Jörn: The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary p [...]. In: BMC Genomics. Jg.8 H. 1. 2007
Inhalt
- Background
- Results
- The growth behavior of both a sigB-deficient and sigB- proficient C. glutamicum strains was comparable under controlled cultivation conditions in glucose-limited batch fermentations
- The C. glutamicum sigB gene encoding an alternative sigma factor was preferentially transcribed during transition from exponential growth to stationary phase
- Global gene expression of a sigB-proficient and a sigB- deficient C. glutamicum strain differed significantly during transition phase
- Classification of genes differentially transcribed only in the sigB-proficient C. glutamicum strain
- Mapping of promoters in front of genes showing an elevated expression in the sigB-proficient C. glutamicum strain at transition phase
- Expression profiles of genes showing an elevated expression in the sigB-proficient C. glutamicum strain at transition phase
- Discussion
- SigB is involved in regulation of transition from exponential growth to stationary phase
- Transition from exponential to stationary growth phase changed the transcription profile of C. glutamicum
- Conclusion
- Methods
- Bacterial strains and growth conditions
- DNA and PCR techniques
- Construction of the sigB deletion mutant C. glutamicum CL1
- Total RNA isolation from C. glutamicum cells for DNA microarray hybridization
- Real-time RT-PCR assays
- RACE-PCR assay for the identification of transcriptional start sites
- Authors' contributions
- Acknowledgements
- References