Sidhu, Vishaldeep K.; Vorhölter, Frank-Jörg; Niehaus, Karsten; Watt, Steven A.: Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. In: BMC Microbiology. Jg.8 H. 1. 2008, S. 87 ff.
Inhalt
- Results
- OMVs are released from the outer membrane of Xcc during growth
- The OMV proteome of Xcc contains outer membrane and virulence-associated proteins
- OMVs do not contain all high abundant proteins present in the OM proteome
- The growth medium has an influence on the OMV protein composition
- Discussion
- Outer membrane vesicles are released from the bacterial surface and contribute membrane proteins to the extracellular proteome
- The OMV proteome does not contain all high abundant outer membrane proteins
- OMVs isolated from M9 and XVM2 medium contain known virulence-associated proteins
- Conclusion
- Methods
- Bacterial Strains and Growth Conditions
- Isolation of OMVs from culture supernatants
- Hot-phenol extraction of LPS
- Crude LPS extraction from whole cells and OMVs
- LPS-specific silver stain
- Isolation of outer membrane proteins (OMPs)
- SDS-PAGE Electrophoresis
- In-gel tryptic digest of proteins
- Nano-LC-ESI-MS/MS measurement trypsin digested gel sections
- MALDI-TOF-MS measurements
- N-terminal secretion signal-peptide prediction using the Signal P software
- Electron Microscopy of OMVs with gold-labelled anti-Xcc antibodies
- Authors' contributions
- Additional material
- Acknowledgements
- References